CBNS108 Discussion on Immunolabeling,2010-1

CBNS108 Discussion on Immunolabeling,2010-1 - Darcie...

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Unformatted text preview: Darcie McClelland 3-31-2010 A labeling technique which makes use of antibody-antigen complexes to mark biological molecules or structures. Immunofluorescence (labeled with fluorescent dye) Immunohistochemistry (labeled with enzyme) Radioimmunolabeling (labeled with radioactive element) Immunofluorescence Immunohistochemistry Antigen : A substance that stimulates the production of an antibody when introduced into the body Source of antigens: toxins, bacteria, foreign blood cells, or cells of transplanted organs Stimulates lymphocytes to produce antibodies Antigenicity of a protein is determined by both sequence of amino acids and by its conformation. May be introduced into an animal by ingestion, inhalation, contact with skin, or more regularly by injection into the bloodstream, skin, peritoneum, or other body part Epitope (antigenic determinant): A localized region on the surface of an antigen that is capable of eliciting an immune response and of combining with a specific antibody to counter that response. Antibody (immunoglobulins): Protein produced by the immune system in response to the presence in the body of antigens. Formed in lymphoid tissues by B cells Found principally in blood serum Specific reactivity with the corresponding antigen. normally absent at birth IgA (~15%): mucous secretions and Colostrum. &#2; IgD (<1%): lymphocyte activity in the cell walls of many B-cells &#2; IgE (<1%): Mediator in allergic responses. &#2; IgG (~75%): stimulates phagocytic cells, activates the complement system, binds neutrophils, and can neutralise toxins. The only antibody that can cross the placenta and confer immunity on the fetus. Subclasses (allotype)in mice: IgG1, IgG2a, IgG2b, IgG3....
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This note was uploaded on 10/31/2010 for the course CBNS 108 taught by Professor Green,demason during the Spring '10 term at UC Riverside.

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CBNS108 Discussion on Immunolabeling,2010-1 - Darcie...

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