CBNS108 Discussion, DNA technology, 2009

CBNS108 Discussion, DNA technology, 2009 -...

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CBNS 108 Discussion #3 DNA techniques Darcie McClelland April 14, 2009
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DNA techniques  Genetic basis of life Why we need to study DNA DNA related techniques Cloning genes PCR Electrophoretic Analysis of DNA   Gene sequence analysis  Complementarity and Hybridization  Northern, Southern, and Western blots  DNA Microarrays  Transgenics
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Genome and DNA 
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Transcription and Translation DNA mRNA Protein Transcription Translation
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DNA cloning Recombinant DNA technology , DNA cloning molecular cloning ,  or gene cloning The transfer of a DNA fragment of interest from  one organism to a self-replicating genetic element  (bacterial plasmid).  Need  donor DNA Vector (plasmid) restriction  enzyme , and  host cells .
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General Steps of Cloning Isolate DNA Cut DNA:  vector and DNA to be cloned are digested  with restriction enzymes to generate complementary  ends  Join DNA:  ligate the foreign DNA into the vector with 
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This note was uploaded on 10/31/2010 for the course CBNS 108 taught by Professor Green,demason during the Spring '10 term at UC Riverside.

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CBNS108 Discussion, DNA technology, 2009 -...

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