Lecture 3 - Polarity in Plant Growth and...

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Polarity in Plant Growth and Development/Embryogenesis OUTLINE A. Expression of Polarity in the Plant Body 2. globular stage 1. Organ growth – stems, roots, leaves 3. heart-stage 2. Embryogenesis 4. torpedo stage 3. Vascular cambium 5. mature embryo 4. Single cells – pollen tubes, root hairs 6. fate map 7. mutants B. Role of Auxin Gradients 1. Generation of auxin gradients a. influx and efflux carriers b. targeting of efflux carrier (PIN) c. origin of auxin d. role in initiation of new organs 2. Auxin response genes a. ARF – Auxin Response Factor b. Aux/IAA co-factors c. Proteosome degradation of co-factor C. Embryogenesis 1. Zygote Reading Assignment : Pages 228-234. Review Reading : Biology, Chapter 35- pages on auxin.
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Study Questions for Lecture 16 1. What is a morphogen and why is the plant hormone auxin considered to be a morphogen? 2. Where is auxin made in plants and what is the mechanism of its transport? 3. Most AUX/IAA mutations affect the stability domain (II) rendering it stable in the presence of auxin. Using the model discussed in class of how auxin regulates gene transcription, describe why these mutations result in auxin insensitivity. 4. Describe the morphological stages of plant embryogenesis and events that occur during each stage. How is auxin involved in the regulation of embryogenesis?
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proximodistal centrolateral centrolateral adaxial adaxial abaxial abaxial Organ Polarity root leaf
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Arabidopsis embryogenesis Box 6A
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Vascular Cambium Phloem production Xylem production
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Cell Polarity Direction of growth pollen tubes and root hairs
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carrier (not shown) is called AUX1. Auxin efflux
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Lecture 3 - Polarity in Plant Growth and...

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