Antigonadal - Materials and Methods Djunarian hamsters,...

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Materials and Methods
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Djunarian hamsters, raised in 16L:8D, ranging from 15-17g were used. The subject animals we pinealectized and cannulated. The experiments examined the effects of infusion solutions and different photo periods.
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Pinealectomy After a head shaving, the animal was put under anesthesia and mounted on a Kopf stereotxic apparatus. The cranium was exposed under a midline incision, while removing bone from the skull. The superficial pineal gland was removed, the wound was covered with gelatin foam, and the skin was clipped together. Sham operations were not
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Cannulation The cannula, a thin tube used for infusions, of 1m length was threaded through and glued to a hypodermic collar. The distal end of the tubing connected through the scruff to the head incision. The cannula enables the efflux of hormone solution to circulate within the animals brain.
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Infusions and Photoperiods Animals received food and water ad  libitum. The cannula was attached to a 1mL
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This note was uploaded on 10/28/2010 for the course ANTHRO 2/AC taught by Professor Wilkes during the Spring '09 term at University of California, Berkeley.

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Antigonadal - Materials and Methods Djunarian hamsters,...

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