Antigonadal - connected through the scruff to the head...

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Click to edit Master subtitle style 10/29/10 Materials and Methods
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10/29/10 Djunarian hamsters, raised in 16L:8D, ranging from 15-17g were used. The subject animals we pinealectized and cannulated. The experiments examined the effects of infusion solutions and different photo periods.
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10/29/10 Pinealectomy After a head shaving, the animal was put under anesthesia and mounted on a Kopf stereotxic apparatus. The cranium was exposed under a midline incision, while removing bone from the skull. The superficial pineal gland was removed, the wound was covered with gelatin foam, and the skin was
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10/29/10 Cannulation The cannula, a thin tube used for infusions, of 1m length was threaded through and glued to a hypodermic collar. The distal end of the tubing
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Unformatted text preview: connected through the scruff to the head incision. The cannula enables the efflux of hormone solution to circulate within 10/29/10 Infusions and Photoperiods Animals received food and water ad libitum. The cannula was attached to a 1mL polstyrene syringe filled with melatonin solutions or saline. In experiments where photoschedules were other than 16L:8D, the time of lights off was 10/29/10 Experiments 1: This experiment was concerned with what the effective dose of melatonin was; solutions were infused for 12h ranging .4ng to 5g. Either a 16L:8D was used or animals were transferred to 10L:14D. 2: Measured how many days would be effective for melatonin infusion: 4, 8, or 12 days, daily dose constant at...
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This note was uploaded on 10/28/2010 for the course ANTHRO 2/AC taught by Professor Wilkes during the Spring '09 term at University of California, Berkeley.

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Antigonadal - connected through the scruff to the head...

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