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methods - For the second part of the experiment red algae...

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Light grown barley leaves and dark grown barley leaves are grinded with methanol and the extract is used for spotting . Spotting was done on the plastic backing sheet. After spotting every time, it was dried with a air hose, and spotted 15 times until there was a clear, dark spot. Then, the sheet was put it in ethyl acetate, and we observed the spot travel by capillary action. The rest of the sample was analyzed in the spectrophotometer.
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Unformatted text preview: For the second part of the experiment, red algae, brown algae, green algae, cyanobacteria, dark grown barley, and light grown barley extracts were mixed with phosphate buffers, and analyzed in the spectrophotometer. Light grown barley and dark grown barley extracts were spinned in the micro centrifuge, and TLC was repeated....
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