Bacteria_Lab 4_2010_student - Lab 4 Bacteriology Lab Topic...

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Lab 4: Bacteriology Lab Topic 5 pp. 67-83
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3 Domain System
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Domain Bacteria, Kingdom Bacteria Most are ~1 µm in size No membrane-bound organelles DNA contained in single circular molecule Mostly unicellular Reproduce via binary fission Some are motile
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Classification of Bacteria Based on: Colony morphology Cell shape aerobic, anaerobic , facultative anaerobe autotrophic , heterotrophic flagellated, non-motile Pathogenic, mutualistic, commensal AND …. Cell wall!
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A. Colony morphology Colony: consists of millions of cells, grown from a single cell 1. Size 2. Shape 3. Margin 4.Surface: Smooth, concentric, wrinkled, etc. 5. Color: yellow, white, beige
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B. Cell Shape (Morphology of Individual cells) spherical (coccus), rod-shaped (bacillus), and spiral-shaped (vibrio, spirillum, spirochaeta) Examine prepared slide of 3 bacteria shapes Perform simple stain to visualize plaque, a mixture of food particles and bacteria from your teeth Streptococcus mutans Lactobacillus acidophilus
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Staphylococcus epidermidis Streptococcus pneumoniae Bacillus anthracis Escherichia coli (E. coli) Examples
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C. Gram Stain: based on cell wall
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  • Fall '07
  • staff
  • Bacteria, Gram stain, C. Gram Stain

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