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Unformatted text preview: Bio-110 Lab 5 1 Bio-110 Laboratory Exercise #5 – Light Microscope II Plant and animal cells Background : In today’s lab you will prepare slides yourself and observe plant and animal cells and also some representatives of the kingdom Protista. A. Plant cells – onion wet mount slide 1. With a scalpel or your fingers, strip a thin, transparent layer of cells from a piece of onion. 2. Place it gently on a clean, dry slide. 3. Add one drop of methylene blue stain and cover with a coverslip. 4. Caution: methylene blue will stain clothes and skin. 5. Observe under the microscope using low and high power and draw a few representative cells. 6. Label the cell wall, cytoplasm, and nucleus. 7. Wash the cover slip and the glass slide with warm water and soap and put it back into the slide box. Low power High power Bio-110 Lab 5 2 B. Plant cells – Elodea wet mount slide (water) 1. Prepare a wet mount of an Elodea leaf with water. 2. To do this, place a drop of water in the middle of the glass slide. 3. Using forceps, remove a small leaf from the tip of an Elodea plant and lay it flat in the drop of water. 4. Cover the leaf with a cover slip....
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