This preview shows page 1. Sign up to view the full content.
Unformatted text preview: mol/10 6 pmol x 10 6 l/L = 20 M 5. What is the recognition site for each restriction enzyme you used (look this up at www.neb.com)? When the restriction enzyme cuts the DNA, what will the ends look like? Does it leave a blunt end, a 5 overhang, or a 3 overhang? Draw this out. NdeI leaves 5 overhangs as do all the enzymes we used: 5CATATG3 5CA3 3GTATAC5 3 GTAT5 6. How would you make 150 ml of a 1.2% agarose gel? 1.2% = 1.2 g / 100 ml 1.2g x 150/100 = 1.8 g 1.8 g of agarose in 150 ml of 1x TAE, boil, cool, pour 7. What sized PCR product do you see? What is the size of the digested plasmid vector?...
View Full Document
This note was uploaded on 11/05/2010 for the course BIOS 4114 taught by Professor Schluchter during the Fall '09 term at UNO.
- Fall '09
- molecular biology