BIO 211 Online 2a

BIO 211 Online 2a - BIO 211 Microbiology Amy Forkey Dr....

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BIO 211 – Microbiology Amy Forkey Dr. Helene Paxton Salem Community College. Online assignment #2a – Bacterial Case studies You may use any material necessary to answer these questions – the text, the internet, etc. The notes and experience that you have gained in the laboratory, as well as the laboratory reports themselves will GREATLY aid you in completing this assignment. Good luck! Please turn in this assignment on paper not on the portal. 1. A patient enters the emergency room at Virtua Memorial hospital showing a range of minor skin irritations that range from severe pimples to more serious boils and several painful absesses around a wound in their arm. The patient tells you that he initially cut his arm a week ago, and the wound became infected and the skin surrounding his wound began to show these symptoms. Based on these symptoms, you begin to suspect that the patient’s wound has become infected with a species of Staphylococcus . In order to confirm this bacterial infection, you take a specimen from the patient in order to perform several tests. Describe would you expect to see for the following tests. a. You stain the bacteria with methylene blue. Do you expect the bacteria cells to be stained with this dye? Why or why not? Yes, the bacterial cells would retain this stain because when you use this dye it highlights the microorganisms to determine cellular shapes and arrangements, also because of the high amount of peptidoglycan in the cell wall. Gram-positive cell walls lack the outer membrane. What shape would the bacteria be? The bacteria would be spherical. b. You stain the specimen with nigrosin. Do you expect the bacteria cells to be stained with this dye? Why or why not? Yes, it can be stained with this dye, because nigrosin is an acidic dye
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This note was uploaded on 11/05/2010 for the course BIOL 211 taught by Professor Paxton during the Fall '10 term at Salem College.

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BIO 211 Online 2a - BIO 211 Microbiology Amy Forkey Dr....

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