AMPylation

AMPylation - AMPylation of Rho GTPases by Vibrio VopS...

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DOI: 10.1126/science.1166382 , 269 (2009); 323 Science et al. Melanie L. Yarbrough, Signaling Disrupts Effector Binding and Downstream VopS Vibrio AMPylation of Rho GTPases by www.sciencemag.org (this information is current as of March 26, 2009 ): The following resources related to this article are available online at http://www.sciencemag.org/cgi/content/full/323/5911/269 version of this article at: including high-resolution figures, can be found in the online Updated information and services, http://www.sciencemag.org/cgi/content/full/1166382/DC1 can be found at: Supporting Online Material found at: can be related to this article A list of selected additional articles on the Science Web sites http://www.sciencemag.org/cgi/content/full/323/5911/269#related-content http://www.sciencemag.org/cgi/content/full/323/5911/269#otherarticles , 8 of which can be accessed for free: cites 14 articles This article http://www.sciencemag.org/cgi/content/full/323/5911/269#otherarticles 1 articles hosted by HighWire Press; see: cited by This article has been http://www.sciencemag.org/cgi/collection/microbio Microbiology : subject collections This article appears in the following http://www.sciencemag.org/about/permissions.dtl in whole or in part can be found at: this article permission to reproduce of this article or about obtaining reprints Information about obtaining registered trademark of AAAS. is a Science 2009 by the American Association for the Advancement of Science; all rights reserved. The title Copyright American Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005. (print ISSN 0036-8075; online ISSN 1095-9203) is published weekly, except the last week in December, by the Science on March 26, 2009 www.sciencemag.org Downloaded from
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AMPylation of Rho GTPases by Vibrio VopS Disrupts Effector Binding and Downstream Signaling Melanie L. Yarbrough, 1 Yan Li, 2,3 Lisa N. Kinch, 4 Nick V. Grishin, 4 Haydn L. Ball, 2,3 Kim Orth 1 * The Vibrio parahaemolyticus type III effector VopS is implicated in cell rounding and the collapse of the actin cytoskeleton by inhibiting Rho guanosine triphosphatases (GTPases). We found that VopS could act to covalently modify a conserved threonine residue on Rho, Rac, and Cdc42 with adenosine 5 -monophosphate (AMP). The resulting AMPylation prevented the interaction of Rho GTPases with downstream effectors, thereby inhibiting actin assembly in the infected cell. Eukaryotic proteins were also directly modified with AMP, potentially expanding the repertoire of posttranslational modifications for molecular signaling. V ibrio parahaemolyticus is a Gram- negative halophilic bacterium that causes foodborne illness worldwide, and in- fections with it lead to acute gastroenteritis ( 1 ). Sequencing of a pathogenic strain of V. parahaemolyticus
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This note was uploaded on 11/01/2010 for the course PRC 1234 taught by Professor All during the Spring '10 term at HKU.

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AMPylation - AMPylation of Rho GTPases by Vibrio VopS...

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