13 Fiber Types EXB101 F10

13 Fiber Types EXB101 F10 - EXB 101 Exercise Physiology...

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EXB 101: Exercise Physiology Lecture 13 Muscle Fiber Types
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KEY POINT: The individual muscle fibers within a muscle are not homogeneous (i.e. they are not all the same) Muscle fibers are classified into different types based on a variety of metabolic and contractile properties
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Fiber Typing Muscle fiber typing is often done by using enzyme histochemical procedures on thin cross-sections of muscles. – The intensity of staining is used as a measure of the activity of various enzymes . The first classifications schemes were based on the staining for myosin ATPase activity – Slow or type I – Fast or type II Fast fibers were divided into subtypes (A, B, C, etc)
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Muscle Fiber Types mATPase acid preincubation mATPase basic preincubation
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Fiber Typing Muscle fiber typing can also be classified based on their oxidative and glycolytic capacities. Muscle cross-sections are stained for myosin (m) ATPase (fast vs slow), Succinate Dehydrogenase (SDH), (an oxidative enzyme), and α -glycerol phosphate
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