Enzyme Kinetics F10

Enzyme Kinetics F10 - Ping-Pong Mechanism Competitive...

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Enzyme Kinetics Prof. W. L. Kelly CHEM 4511/6501 Fall 2010
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Enzyme Kinetics I. Chemical Kinetics II. Enzyme Kinetics II. isubstrate Reactions III. Bisubstrate Reactions IV. Enzyme Inhibition V. Control of Enzyme Function VI. Drug Design Parameters
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Chemical and Enzyme Kinetics or a unimolecular reaction: S P k For a unimolecular reaction: V = d[P] dt k[S] = d[S] dt = -
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Chemical and Enzyme Kinetics or an enzyme- atalyzed reaction with one substrate: E + S E + P For an enzyme catalyzed reaction with one substrate:
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Chemical and Enzyme Kinetics E + S E•S E•P E + P k 1 2 3 -1 -2 -3
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The Steady State Approximation in Enzyme Kinetics k E + S E•S E + P 1 2 -1 [E T ] = [E] + [ES] V = d[P] dt cat [ES] = 1 [E][S] = k cat [ES] + k -1 [ES] [ES] = 1 [E T ][S] cat + k -1 + k 1 [S] V = cat [E T ][S] cat + k -1 1 + [S] ( )
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The Michaelis-Menten Equation V = k cat [E T ][S] cat + k -1 + [S] ( ) 1 V = V max [S] K M + [S]
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Examples of Kinetic Parameters
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Lineweaver-Burk Plot V = V max [S] K M + [S] 1 = V ax M [S] + 1 V ax 1 ( ) V max max
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Bisubstrate Reactions equential Reaction: Sequential Reaction:
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Bisubstrate Reactions
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Unformatted text preview: Ping-Pong Mechanism Competitive Inhibition Michaelis-Menten Equation: Adenosine Deaminase Uncompetitive Inhibition Michaelis-Menten Equation: Mixed Inhibition Michaelis-Menten Equation: Mechanism-Based Inactivation: Ornithine Decarboxylase Allosteric Regulation Aspartate Transcarbamoylase Blue and purple: catalytic subunits Red and Yellow: regulatory subunits Aspartate Transcarbamoylase Reversible Covalent Modification to Regulate Activity Glycogen Phosphorylase Proteolytic Regulation Transpeptidase and β-Lactam Antibiotics Lazar, K.; Walker S. Curr. Opin. Chem. Biol . 2002 , 6 , 786-793. Fischer, J.F.; et al . Chem. Rev . 2005 , 105 , 395-424. Transpeptidase and β-Lactam Antibiotics Overcoming the hurdles… R = H Ampicillin R = OH Amoxicillin Clavulanic Acid: HIV Protease Inhibitors...
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This note was uploaded on 11/04/2010 for the course CHEM 6501 taught by Professor Wendyl.kelly during the Fall '10 term at Georgia Tech.

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Enzyme Kinetics F10 - Ping-Pong Mechanism Competitive...

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