Proteins I_slides_F10

Proteins I_slides_F10 - Sandwich ELISA: Immunoblot (aka...

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Proteins Prof. W. L. Kelly CHEM 4511/6501 Fall 2010
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Proteins I. Polypeptides, Proteins, and Primary Structure I rotein Purification and Analysis II. Protein Purification and Analysis III. Protein Sequencing IV. Secondary Structure V. Tertiary Structure VI. Quaternary Structure VII. Protein Stability VIII. Postranslational Modifications IX. Bioinformatics X. nf m
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Proteins
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Amino Acid Occurrence in Proteins Amino Acid Gly Avg Occurrence in Proteins (%) 7.2 y Ala Val Leu Ile et 7.8 6.6 9.1 5.3 2 Met Pro Phe Trp Ser 2.2 5.2 3.9 1.4 6.8 Thr Asn Gln Tyr ys 5.9 4.3 4.3 3.2 9 Cys Lys Arg His Asp 1.9 5.9 5.1 2.3 5.3 Glu 6.3
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Primary Structure et- ly- le- al- lu- ln- ys- ys- la- er- al- rp- la
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II. Protein Purification
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Activity vs. Specific Activity
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Activity Assays
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Enzyme-Linked Immunoabsorbent Assay (ELISA): Immunochemical Assays
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Unformatted text preview: Sandwich ELISA: Immunoblot (aka Western Blot): Absorbance Spectroscopy to Quantify a Protein Bradford Assay to Quantitate Protein Protein Purification Centrifugation Ammonium Sulfate Precipitation Column Chromatography Ion Exchange Chromatography e.g. Cation Exchange Size Exclusion Chromatography Affinity Chromatography SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE) SDS-PAGE Isoelectric Focusing Two-Dimensional Gel Electrophoresis Protein Sequencing Insulin rederick Sanger Frederick Sanger Protein Sequencing Protein Sequencing Breaking Disulfide Bonds alkylation Alkylated Proteases Cleavage of Protein and Assembly of Sequence Electrospray Ionization Mass Spectrometry (ESI-MS) Solid Phase Peptide Synthesis...
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Proteins I_slides_F10 - Sandwich ELISA: Immunoblot (aka...

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