Protein Fx_PS6_F10 - CHEM 4511/6501 Problem Set 6 1....

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CHEM 4511/6501 Problem Set 6 1. Estimate K d from the following data describing a ligand binding to a protein. [Ligand] (mM) θ 0.25 0.30 0.50 0.45 0.80 0.56 1.4 0.66 2.2 0.80 3.0 0.83 4.5 0.86 6.0 0.93 2. Which set of binding data is likely to represent cooperative ligand binding to an oligomeric protein? (a) (b) [Ligand] (mM) θ 0.1 0.3 0.2 0.5 0.4 0.7 0.7 0.9 3. Protein A has a binding site for ligand X with a K d of 10 -6 M. Protein B has a binding site for ligand X with a K d of 10 -9 M. A. Which protein has a higher affinity for ligand X? Explain your reasoning. B. Convert K d to K a for both proteins. 4. An antibody binds to an antigen with a K d of 5 x 10 -8 M. At what concentration of antigen will θ be: A. 0.2. B. 0.5 C. 0.6 D. 0.8 5. In humans, the urge to breathe results from high concentrations of CO 2 in the blood; there are no direct physiological sensors of blood pO 2 . Skindivers often hyperventilate (breathe rapidly and deeply for several minutes) just before making a dive in the belief
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This note was uploaded on 11/04/2010 for the course CHEM 6501 taught by Professor Wendyl.kelly during the Fall '10 term at Georgia Institute of Technology.

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Protein Fx_PS6_F10 - CHEM 4511/6501 Problem Set 6 1....

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