Proteins II_PS5_F10 - CHEM 4511/6501 Problem Set 5 1. In...

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CHEM 4511/6501 Problem Set 5 1. In site-directed mutagenesis experiments, Gly is often successfully substituted for Val, but Val can rarely substitute for Gly successfully. Explain. 2. Below is a list of the first 10 residues of the B helix in myoglobin from different organisms. Position 1 2 3 4 5 6 7 8 9 10 Human D I P G H G Q E V L Chicken D I A G H G H E V L Alligator K L P E H G H E V I Turtle D L S A H G Q E V I Tuna D Y T T M G G L V L Carp D F E G T G G E V L Based on this information, which positions (a) appear unable to tolerate substitutions, (b) can tolerate conservative substitution, and (c) are highly variable? 3. Explain why gelatin, which is mostly collagen, is nutritionally inferior to other types of protein. 4. You are performing site-directed mutagenesis to test predictions about which residues are essential for a protein’s function. Which of each pair of amino acid substitutions listed below would you expect to disrupt protein structure the most? Explain. A. Val replaced by Ala or Phe.
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This note was uploaded on 11/04/2010 for the course CHEM 6501 taught by Professor Wendyl.kelly during the Fall '10 term at Georgia Institute of Technology.

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Proteins II_PS5_F10 - CHEM 4511/6501 Problem Set 5 1. In...

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