PS7 4511 F10 - P) with a negative G rxn . ( B ) The...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
CHEM 4511/6501 Problem Set 7 1. Voet, Voet and Pratt Chapter 11:1, 2, 4, 5, 7, 8, 9, 12, 13, 16, 18 2. RNaseA is an enzyme that catalyzes the cleavage of RNA by the following mechanism in which both histidine residues have pK a ’s near 6. (A) Using the axis above, draw what you expect the pH-rate profile to look like for this reaction: (B) Why is RNaseA unable to catalyze the hydrolysis of DNA? 3. α -Galactosidase catalyzes the reaction shown below by a reaction similar to that of lysozyme. Compound I is a potent inhibitor (but not a substrate) of α -galactosidase. Identify a structural feature of compound I that helps it to be a potent inhibitor of α - galactosidase. O O P O Base RNA-O O O O H NN H RNA N H N H O Base RNA-O OH NN H RNA N H N O P O O O H rate pH O O OH OH HO HO R O OH OH OH HO HO +HOR NH NH R NH OH HO HO I
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4. Draw a transition state diagram (reaction coordinate diagram) for ( A ) A nonenzymatic reaction (S
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: P) with a negative G rxn . ( B ) The corresponding enzyme-catalyzed reaction in which S binds loosely to the enzyme. ( C ) The corresponding enzyme catalyzed reaction in which S binds very tightly to the enzyme. A B C Indicate G in each case ( A-C ). For B and C , are they larger or smaller than for A ? (Answer both) Do you expect reaction B and C will be slower or faster than A ? (Answer both) 5. Haloalkane dehalogenase is a detoxification enzyme that catalyzes the reaction below. This enzyme has an aspartate residue that is essential for catalysis. Propose a mechanism for the enzyme-catalyzed reaction be sure to include a role for the aspartate residue . G Reaction Progress G Reaction Progress G Reaction Progress Cl Cl OH Cl + H + + Cl-+ H 2 O Haloalkane Dehalogenase...
View Full Document

Page1 / 2

PS7 4511 F10 - P) with a negative G rxn . ( B ) The...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online