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Unformatted text preview: Experiment #7 Isolation of Components of Spearmint Oil and Thin Layer Chromatography 10/15/09 Important Structures: 1 Procedure: First, a TLC of spearmint oil was taken alongside its components: Carvone, Limonene, and Dihydrocarveol, in order to use as a standard. Then several drops of spearmint oil were separated into its components by column chromatography through 1g of silica gel. The first two 2ml elutions were done by adding pure hexane. The next three 2ml elutions were done with a 5% ethyl acetate/hexane solution, and the last three 2ml elutions were done with a 10% ethyl acetate/hexane solution. A TLC plate was then divided into eighths and spotted with each elution in order to find which samples contained the components of interest. Elutions that were identified as containing a component were then run through individual TLCs alongside with the suspected component as a comparable standard (apparently empty elutions were spotted as well in order to double check). An IR of Carvone was provided by the TA as an example of a pure substance to show groups that would be present and/or absent in a relatively pure sample elution. Results: *See attached IR Spectrum TLC Plates: All three substances showed with KMnO4 staining because any compound with an alkene group will reduce maroon KMnO4 to brown MnO2....
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