Effect of Light Intensity on Photosynthesis

Effect of Light Intensity on Photosynthesis - Effect of...

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Effect of Light Intensity on Photosynthesis ( : ) Lab 19 Measuring Photosynthetic Rate Introduction The objective of this experiment is to perform a spinach leaf disk assay in order to measure photosynthetic rate under varying . , . experimental conditions Specifically the role of light intensity in the process of photosynthesis will be investigated By utilizing varying , , , numbers of Kimwipe sheets placed between a light source and the leaf disks different degrees of shades will be made and thus we will . , study our question of interest in how light intensity affects photosynthesis In photosynthesis plant cells convert light energy into chemical . energy that is stored in sugars and other organic compounds The overall reaction of photosynthesis can be summarized by the following : equation 6CO 2 + 6H 2 --> O C 6 H 12 O 6 + 6O 2 : , . It is a process consisting of two stages the light reactions and the Calvin cycle also referred to as the dark reactions In the , , . light reactions light is absorbed by chlorophyll molecules exciting their electrons The energy of these excited electrons is then used to . , + , join ADP and inorganic phosphate to form ATP In addition NADP combines with the excited electrons to form NADPH which can be . , considered as a temporary shuttle storing the energized electrons In the process water taken from the environment is split and oxygen is - . , released into the atmosphere as a by product In the Calvin cycle carbon from carbon dioxide taken from the environment combines with , , the energy from ATP and electrons from NADPH which were the resulting outputs of the previous light reactions to produce sugar . , molecules While the null hypothesis is that photosynthetic rate will not be affected as the degree of shading increases the alternative , hypothesis is that photosynthetic rate will decrease as degree of shading increases based on previous knowledge that light energy is what . drives the reactions of photosynthesis to occur
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Methods The protocol for the spinach leaf disk assay can be found in Biology 181
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Effect of Light Intensity on Photosynthesis - Effect of...

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