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Random fun Facts - Sources of protein are usually tissue or microbial cells Protein purification create a crude extract by breaking up the cells

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Sources of protein are usually tissue or microbial cells Protein purification: create a crude extract by breaking up the cells you have (either tissue or microbial cells), releasing the proteins into the extract. Differential centrifugation can separate subcellular fractions, or isolate specific organelles. Fractionation – separating proteins on the basis of size or charge - Protein solubility is a complex function of pH, temperature, salt concentration, and other factors. - High salt concentrations lower protein solubility - Adding salt in the right amount can selectively precipitate some proteins. Dialysis – procedure that separates proteins from solvents by taking advantage of the proteins’ larger size. - Partially purified extract is placed in a bag made of a semipermeable membrane. - When the bag is suspended in a larger volume of buffered solution, the membrane allows the exchange of salt and buffer but not proteins . Dialysis retains large proteins within the membranous bag while allowing the concentration of
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This note was uploaded on 11/07/2010 for the course CBN 356 taught by Professor Merill during the Spring '08 term at Rutgers.

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