AbsorptionSpectroscopyChem152.Fall2009 - Absorption...

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Absorption Spectroscopy Author: Miyoung Chung Lab Partner: Jonathan Siquieros Instructor: Stephanie Tolbert Chemistry 152, Section 014 Date Work Performed: August 25, 2009 Date Work Submitted: September 1, 2009 Abstract: Using Red Food Dye with a concentration of 2.777 x 10 -4 M, the absorbance values were found by using a spectrometer to determine the wavelength at maximal absorbance. The absorbances of different diluted solutions were found and graphed against concentration in order to find Epsilon in Beer’s Law. Epsilon was found to be 173.31 M -1 cm -1 .
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Chung 1 Introduction: The purpose of the Absorption Spectroscopy experiment was to measure absorbance of a given food dye and find the concentration using Beer’s Law (A = εbC). Given the values for volume and finding Epsilon, the concentration can be calculated. The best wavelength for the given dye was found and used to find the absorbance in different samples of known concentration. Using serial dilution, the absorbance of these different solutions were found and used to make a calibration plot. The calibration plot will allow for the determination of concentration given a certain absorbance. Materials and Method:
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AbsorptionSpectroscopyChem152.Fall2009 - Absorption...

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