Chemical Separation Lab 2

Chemical Separation Lab 2 - clinging along the plate. We...

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Chemical Separation Author: Miyoung Chung Instructor: Jared Boulds Chemistry 151, Section 003 Date Work Performed: June 11, 2009 Date Work Submitted: June 15, 2009 ABSTRACT
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Chung 1 Introduction It is crucial in science to separate substances in order to understand their properties and utilize them in other applications. Compounds can be separated by taking advantage of their differences in attraction. We performed this type of separation with chromatography. By breaking down the cell walls of spinach leaves, we extracted the pigment by mixing it with hexane and acetone. Using this solution as the mobile phase, we put this solution onto a TLC strip using it as the stationary phase. The different pigments in the spinach leaf will have different mobile phases that travel at different rates. Different pigments include cholophylls and cartenoids 1 . As the solution travels up the TLC plate, the pigments will separate, essentially
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Unformatted text preview: clinging along the plate. We can account for the different pigments by comparing the retention factors, which we will refer to as Equation 1 (R f = Distance 1 /Distance 2 ) 1 . Results TLC Strip Plate Results Table #1 Color Trial 1 (cm) Trial 2 (cm) Trial 3 (cm) Average (cm) Standard Deviation Calculated R f Yellow 1 7.6 8.2 7.5 7.8 0.4 0.93 Green 1 5.9 6.0 6.6 6.2 0.5 0.74 Green 2 5.7 6.2 3.9 3.4 0.46 Yellow 2 2.6 2.2 3.5 2.8 0.7 0.33 Solvent Front 8.1 8.5 8.6 8.4 0.3 n/a Calculations-Yellow 1 R f = Distance 1 /Distance 2 R f = 7.8 cm/8.4cm R f = 0.93 Chung 2-Green 1 R f = Distance 1 /Distance 2 R f = 6.2cm/8.4 cm R f = 0.74-Green 2 R f = Distance 1 /Distance 2 R f = 3.9 cm/8.4 cm R f =0.46-Yellow 2 R f = Distance 1 /Distance 2 R f = 2.8 cm / 8.4 cm R f =0.33 Discussion...
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Chemical Separation Lab 2 - clinging along the plate. We...

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