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Genetics%20final[1] - Yeast 2 Hybrid ClickMariaMaster...

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Click to edit Master subtitle style Yeast 2 Hybrid Maria Grisales, Aamir Mazhar, Bich Vo Genetics Dr. Simmons October 15, 2010
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Click to edit Master subtitle style History - 1989, Stanley Fields pioneered the technique -  First used to detect protein-protein interactions -  Yeast was the model organism
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What is Yeast 2 Hybrid? Used to analyze : n Protein-Protein interactions n Protein-DNA interactions
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What is Yeast 2 Hybrid? n Foreign DNA fragments are inserted in yeast strains in form of plasmids n DNA-binding domain and activation domain fragments combine. n Yeast are mutant and contain the plasmids containing the specific nutrient
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How Does it Work? n First the “bait” protein attaches to DNA n Binding Domain attaches to promoter n Then “prey” protein interacts with “bait” n Activating Domain allows RNA Polymerase II to transcribe reporter gene n Reporter genes allow easy identification of positive colonies n Typically presence of amino acids or color http://cmbi.bjmu.edu.cn/cmbidata/proteome/method /html/2hybrid02.files/Image10.gif
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Video Explanation n http://www.sumanasinc.com/webcontent/anima
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