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Lab Notes - What Is An Enzyme Organic catalyst o Most are...

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Unformatted text preview: What Is An Enzyme? Organic catalyst o Most are proteins o Increases the rate of reaction o Not consumed Main function: to increase chemical reaction rates by lowering the activation energy How Do Enzymes Work? Complex 3-D shape Starts at the active site. Substrate binds to the active site. The enzyme-substrate complex allows the reaction to proceed (Stabilize the transition state). The product separates from the enzyme. The enzyme may now bind to another substrate. Do Different Factors Effect Enzyme Activity— Enzyme concentration pH Substrate concentration Temperature Structure of the substrate Source of the enzyme Lock and Key Model Induced-fit Model Enzyme Kinetics Spectrophotometer What Does I t Do? Detects the amount of radiant energy absorbed. Why Use I t? The light absorbed is proportional to the concentration of the compound. What Is The Relationship Between Absorbance and Concentration? Beer–Lambert law At a given wavelength of light A= lc ε o A= absorbance o = absorptivity (constant for a given substance) ε o l=length of the path (constant for a given instrument o c= concentration OH OH + 1/2O 2 Catechol Oxidase Catechol Benzoquinone O O Standard Curve...
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Lab Notes - What Is An Enzyme Organic catalyst o Most are...

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