301_Lecture_Oct_12

301_Lecture_Oct_12 - Triosephosphate isomerase Irwin Rose...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Dihydroxyacetone Phosphate (DHAP) ( R )-Glyceraldehyde 3-Phosphate (GAP) . E CO 2 - E CO 2 H E CO 2 - + + O OH H H OPO 3 2- O H OH OPO 3 2- OH H H O OPO 3 2- Specific for phosphodianion substrates "Perfect" enzyme Single-base proton transfer mechanism through a cis - enediolate intermediate Stereospecific for R- glyceraldehyde 3-phosphate and the pro-R proton at C1 of dihydroxyacetone phosphate Irwin Rose, Greg Petsko and Jeremy Knowles Irwin Rose, Greg Petsko and Jeremy Knowles Triosephosphate isomerase
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Partitioning of Enediol(ate) in DOD D 2 O Enediol(ate) k ex E-CO 2 D OD H O OD H Enediol(ate) E-CO 2 H OD H H OD O k T 49% OD D H O OD OD D H OH O 21% 31% Intermediate sequestered from solvent at the active site. Biochemistry , 44, 2610-2621 (2005) , 6 , 391-396 (2008).
Background image of page 2
E-CO 2 - O H H H OD HPO 3 2- O(D) H H OD O(D) H H O H E-CO 2 H HPO 3 2- O(D) H H OD E-CO 2 D HPO 3 2- O(D) D H O H O H H D OD k ( iso ) H k ex k ( iso ) D k ( Tr ) D
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
E-CO 2 - 13 C O H H H
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 11/10/2010 for the course CHE Intermedia taught by Professor Dr.richard during the Spring '10 term at SUNY Buffalo.

Page1 / 20

301_Lecture_Oct_12 - Triosephosphate isomerase Irwin Rose...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online