Lecture14 - Summary 1. 2. 3. 4. 5. Nucleating proteins of...

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Summary 1. Nucleating proteins of microtubules and actin filaments; 2. Subunit/monomer binding proteins, severing proteins; 3. Proteins that bind to the side of the filaments; 4. Plus end binding proteins: capping proteins; 5. Higher order organization of three types of cytoskeletal systems; 1. Bundling and gel-forming proteins of actin cytoskeleton; 2. Anchoring of cortical actin to the plasma memebrane.
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Lecture 14 Motor proteins and cytoskeleton-mediated cell behavior
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Myosin II and myosin II bipolar thick filament 2 heavy chains: Globular head domain (ATP) long tails (coiled coils) 2 light chains: Bipolar thick filaments: Tail-tail interactions Hundreds of myosin II molecules Thin filament: actin filament
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Experimental evidence for the motor activity of the myosin head Myosin head S1: by chymotrypsin and papain Attached to the glass slide 0.6 sec apart 4 um/sec when ATP is added
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Myosin superfamily Similar motor domains Move to plus end except VI Small insertion in the motor domain
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Function of myosins Myosin II: contractile activity in muscle and nonmuscle cells, cytokinesis (pinching apart of a dividing cell into two daughters), forward translocation of cell body during migration); Myosin I: contain a second actin-binding or membrane-binding site in their tails- intracellular organization and the protrusion of actin-rich structures at the cell surface; Myosin V: vesicle and organelle transport.
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This note was uploaded on 11/10/2010 for the course BICD BICD 110 taught by Professor Zao during the Spring '09 term at UCSD.

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Lecture14 - Summary 1. 2. 3. 4. 5. Nucleating proteins of...

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