Bios320_Test2Notes

Bios320_Test2Notes - Bio 320 09/16/10 Sterilization- the...

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Unformatted text preview: Bio 320 09/16/10 Sterilization- the desire to eliminate all microorganisms including spores Sanitization/disinfection- inhibit disease causing microorganisms Antiseptic- applied to the surface of tissues to eliminate disease causing microorganisms _____cide- to kill _____static- inhibit effectiveness Effectiveness: Population size Species Do they produce spores Concentration of the killing agent How long it is exposed to the killing agent Temperature of the environment Local parameters (Ph) Physical Control: Heat: Moist heat Autoclave 15 minutes, 121 C, 15 psi Pasteurization 62.8 C for 30 minutes 71.7 C for 15 minutes Tyndallization 100 C- kills all viable cells Incubate at 37 C for one day 100 C- kills all germinated spores Incubate 100 C- just to make sure Incubate Dry Heat: Oven, Incinerator Killing Efficiency TDT: the time required to kill all microorganisms at a defined temperature at defined conditions D Value-Declined Reduction Time: kills 90% of microorganisms By not using the strongest agent possible you dont reduce the time for resistance Low Temperature: Freezing: 0 C Water molecules crystallize reducing the water availability to 0 Refrigeration: 5-10 C Slows microbial growth Filtering: Liquids: Thick fiber (depth)- filter paper Membrane filters- defined by pore size 0.20 microns for microbes Air: HEPA: High efficiency particulate air filter Radiation: Low UV 260 nm Pyrimidine Dimers In DNA, [A-T, G-C, T-T] Gamma Rays Cobalt 60 Chemical Methods: Liquids Phenols: denature proteins Used as surface disinfectants Alcohols: denature proteins Works as a good preservative Ethanol Isopropel Halogens: disrupts tyrosine and destroys amino acids Iodine Chlorine Gas Heavy Metals: denatures proteins Hg Silver Nitrate Arsenic Detergents/Surfactants: disrupts membranes Ammonium Alkaline Aldehydes: destroys proteins Works as a good preservative Formaldehyde Gases: Ethylene Oxide Beta Propiolactone Evaluation of Antimicrobial Agents: Check for slides (not much information given) Phenol Coefficient Test Chemotherapeutic Agents: agents that can be used internally Antibiotics: substances we put in our bodies to rid of microbes VS. Antibodies: proteins produced by cells Paul Ehrlich: Looked at dead and living cells Used a stain call trypan red Discovered it killed trypanosomes Used Arsphenamine Discovered it killed syphilis Bios 320 9/21 Sigma Scientists Gerhard Domagk Worked with Protosil Red Traditionally used to cure leather Antibiotic against Staphalococcus and Streptococcus Bacteria changed Protosil Red into Sulfanilamide Sulfer is very strong antibiotic agent...
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This note was uploaded on 11/10/2010 for the course BIOS 6767 taught by Professor Lee during the Spring '10 term at LA Tech.

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Bios320_Test2Notes - Bio 320 09/16/10 Sterilization- the...

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