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Bios320_Test2Notes

Bios320_Test2Notes - Bio 320 Sterilization the desire to...

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Unformatted text preview: Bio 320 09/16/10 Sterilization- the desire to eliminate all microorganisms including spores Sanitization/disinfection- inhibit disease causing microorganisms Antiseptic- applied to the surface of tissues to eliminate disease causing microorganisms _____cide- to kill _____static- inhibit effectiveness Effectiveness: Population size Species Do they produce spores Concentration of the killing agent How long it is exposed to the killing agent Temperature of the environment Local parameters (Ph) Physical Control: Heat: Moist heat • Autoclave ♦ 15 minutes, 121° C, 15 psi • Pasteurization ♦ 62.8° C for 30 minutes ♦ 71.7° C for 15 minutes • Tyndallization ♦ 100° C- kills all viable cells ♦ Incubate at 37° C for one day ♦ 100° C- kills all germinated spores ♦ Incubate ♦ 100° C- just to make sure ♦ Incubate Dry Heat: Oven, Incinerator • Killing Efficiency ♦ TDT: the time required to kill all microorganisms at a defined temperature at defined conditions ♦ D Value-Declined Reduction Time: kills 90% of microorganisms ♦ By not using the strongest agent possible you don’t reduce the time for resistance • Low Temperature: ♦ Freezing: 0° C Water molecules crystallize reducing the water availability to 0 ♦ Refrigeration: 5-10° C Slows microbial growth Filtering: Liquids: • Thick fiber (depth)- filter paper • Membrane filters- defined by pore size ♦ 0.20 microns for microbes Air: • HEPA: High efficiency particulate air filter Radiation: Low UV • 260 nm Pyrimidine Dimers • In DNA, [A-T, G-C, T-T] Gamma Rays • Cobalt 60 Chemical Methods: Liquids Phenols: denature proteins • Used as surface disinfectants Alcohols: denature proteins • Works as a good preservative • Ethanol • Isopropel Halogens: disrupts tyrosine and destroys amino acids • Iodine • Chlorine Gas Heavy Metals: denatures proteins • Hg • Silver Nitrate • Arsenic Detergents/Surfactants: disrupts membranes • Ammonium • Alkaline Aldehydes: destroys proteins • Works as a good preservative • Formaldehyde Gases: Ethylene Oxide Beta Propiolactone Evaluation of Antimicrobial Agents: Check for slides (not much information given) Phenol Coefficient Test Chemotherapeutic Agents: agents that can be used internally Antibiotics: substances we put in our bodies to rid of microbes VS. Antibodies: proteins produced by cells Paul Ehrlich: Looked at dead and living cells Used a stain call trypan red • Discovered it killed trypanosomes Used Arsphenamine • Discovered it killed syphilis Bios 320 9/21 Sigma Scientists Gerhard Domagk Worked with Protosil Red • Traditionally used to cure leather • Antibiotic against Staphalococcus and Streptococcus • Bacteria changed Protosil Red into Sulfanilamide ♦ Sulfer is very strong antibiotic agent...
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Bios320_Test2Notes - Bio 320 Sterilization the desire to...

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