5 - Lecture 5: Chromatographic separations of proteins...

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Lecture 5: Chromatographic separations of proteins Monday, August 16, 2010
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Almost all current protein purifcation techniques use diFerent ±orms o± chromatography (a process in which complex mixtures o± molecules are separated by many repeated partitionings between a flowing (mobile) phase and a stationary phase). Monday, August 16, 2010
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The general process Monday, August 16, 2010
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There are diferent Forms oF chromatography that exploit diferent properties oF proteins Ion Exchange Chromatography: A process in which ions that are electrostatically bound to a solid support are replaced by ions in solution. The solid support is an insoluble matrix such as cellulose, to which charged functional groups are attached: Monday, August 16, 2010
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The functional group attached to the matrix determines the type of exchanger that it is. ANION EXCHANGE matrices have positively charged groups attached to them: DEAE (diethylaminoethyl) group Monday, August 16, 2010
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CATION EXCHANGE matrices have negatively charged groups attached to them: CM (carboxymethyl) group Monday, August 16, 2010
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The general procedure for an ion-exchange column Monday, August 16, 2010
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5 - Lecture 5: Chromatographic separations of proteins...

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