biochem - Lecture 9: Tertiary and Quarternary Structure of...

Info iconThis preview shows pages 1–9. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Lecture 9: Tertiary and Quarternary Structure of Proteins Friday, August 20, 2010 The tertiary structure of a protein describes how the various elements of secondary structure are packed together to create the overall topology of a protein in its NATIVE state Various representations of myoglobin Friday, August 20, 2010 How do you determine the atomic-level structure of a protein (or any biological macromolecule)? Two methods: X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy Friday, August 20, 2010 X-ray crystallography: First, you must generate ordered CRYSTALS of the protein of interest: Note that this is similar to using ammonium sulfate to precipitate proteins during the initial stages of protein purication. In fact, crystallization is best thought of as a slow, orderly precipitation process! Friday, August 20, 2010 A regular crystalline array (from salt to a protein) diffract radiation is a very specific way, which can be easily observed Friday, August 20, 2010 This generates a DIFFRACTION PATTERN each refection (aka, spot) provides inFormation about the shape oF the electron cloud surrounding the molecule oF interest riday, August 20, 2010 This picture is mathematically manipulated to generate a picture of the electron density, which can then be interpreted in the form of a MODEL Friday, August 20, 2010 Often, these pictures are resolution limited Friday, August 20, 2010 NMR spectroscopy, on the other hand, uses a...
View Full Document

Page1 / 29

biochem - Lecture 9: Tertiary and Quarternary Structure of...

This preview shows document pages 1 - 9. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online