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Unformatted text preview: Name:________________________Student ID#_____________________ Page 1 CHEM 4711/5711 Midterm 2, Oct. 14, 2004 Answer the following questions as succinctly as possible. Use the back of the sheet if you need additional space. MAKE SURE TO PUT YOUR NAME ON ALL PAGES (A TOTAL OF 8 PAGES, 10 QUESTIONS) Question 1: 10 points You are given a recipe for making a MES buffer that involves adding 60 grams of MES (free acid form, pKa = 6.21, M.W.= 195.2 g/mol) and 60 mL of 5 M NaOH to sufficient water to yield a total volume of 0.5 liter. What is the pH of the solution? You must show your work to get full credit. Name:________________________Student ID#_____________________ Page 2 Question 2: 15 points The sequence of crinia-angiotensin, an angiotensin II-like undecapeptide from the skin of the Australian frog is determined. A single round of Edman degradation releases DNP- Ala. A second sample of the peptide is then treated with chymotrypsin. Two gragments are released with the following amino acid compositions: Fragment I ( H, P, F, V ) and...
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