Sem1 Practical3 - Results (Final).docx - BIO20002 – The...

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BIO20002 – The Microbial WorldPractical 3 – ResultsName:Yeong Kah HoID:101211968A)SAMPLE PREPARATION – SERIAL DILUTIONQuestions:The undiluted suspension was denoted as1:1during 2-fold dilution series because1:1signifies “1 part of original solution made into 1 times the volume”. In a similar way, the1:16notation is equivalent to “1 part of original solution made into 16 times the volume”.a)How will you describe a solution (using theX:Ynotation) which had its solvent driedoff to give a final amount which is only 20% of its original volume?
b)Determine the amount (volume) of original solution required if final volume 750mLof1:17dilution is to be prepared.
c)From an undiluted solution, an aliquot of 3.0 mL was taken and diluted to 50 mL.By taking 600 µL of the first dilution and diluting it into 4.0 mL, a second dilutionwas prepared. How diluted (using theX:Ynotation) is the second solution comparedto the undiluted solution?

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Yeong Kah Ho

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