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Unformatted text preview: Aileen Caceres BB2902 Pre-lab questions: These are to be completed and turned in using the prelab assignment. 1. For both parts I and II write a statement of a hypothesis that will be tested. Beta-galactosidase production will be induced by the presence of lactose. 2. Explain what happens when the lac operon is induced by lactose. What happens if glucose is also present? What happens if only glucose is present? The lac operon is induced by the presence of lactose because lactose has the ability to bind to the repressor that inhibits the lac operon. Once it binds to the repressor, it changes its conformation so that it can no longer bind to the operator and can no longer be a repressor. Once the repressor is removed by lactose the RNAP can bind to the promoter region and allow the genes to be transcribed for the synthesis of lactose. If glucose is also present its levels also regulate the lac operon. If there are low levels of glucose, then there is a high level of cAMP. cAMP then binds operon....
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- Spring '08