micro-chap26

micro-chap26 - 26.1 o Genetic engineering allows for the...

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26.1 o Genetic engineering allows for the cloning and expression of eukaryotic genes in prokaryotes. Hormones such as insulin and some other human proteins are now manufactured in this manner. 26.2 o It is possible to achieve very high levels of expression of eukaryotic genes in prokaryotes. However, the expressed gene must be free of Introns. This can be accomplished by using reverse transcriptase to synthesize cDNA from the mature mRNA encoding the protein of interest. It can also be accomplished by making an entirely synthetic gene using knowledge of the amino acid sequence of the protein of interest. Protein fusions are often used to stabilize or solubilize the cloned protein. 26.3 o The first human protein made commercially using engineered bacteria was human insulin, but numerous other hormones and other human proteins are now being produced 26.4 o Many proteins are extremely expensive to obtain by traditional purification methods because they are found in human or animal tissues in only small
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This note was uploaded on 11/16/2010 for the course BIO 3317 taught by Professor Fentisel during the Spring '10 term at Temple.

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micro-chap26 - 26.1 o Genetic engineering allows for the...

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