BIO 221 Exam 2 - E lectron t ransport chain -Prokaryotes...

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Electron transport chain -Prokaryotes located in cytoplasmic membrane -Eukaryotes located in mitochondria membrane -NAD and FAD= transport both (e and H) -Iron-sulfur Protein= transport (e only) heme or cytochrome c -Mitochondrial ETC Protons are by complex I, III and IV that creats the PMF ATP synthase allows protons to reenter matrix, using energy released to drive ATP synthesis -Proton pump is due to quinine (Q) loop Happens in : Complex III- protons from cytoplasm reduce quinone then reduce FeS and then protons pumped outside Bacterial Electron Transport: -uses different transport complexes -uses different terminal electron acceptors -pump variable numbers of H + -can use more than one transport chain at once -may or may not have cytochrome C which is an oxidase test….either uses oxygen or not Chemolithotrophs= get electrons from inorganic energy sources and use them to generate ATP by oxdative phosphorylation H 2 H 2 S Fe 2+ NH 3 HNO 2 Chemoorganotrophs (Get from book)!!!
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Photosynthesis -light energy oxidizes chlorophyll in a reaction center -antenna pigments channel light to reaction center where electrons are released and go to electron transport chain Oxygenic photosynthesis -electrons passed from PSII to PSI make ATP -electrons passed from PSI to NADP reductase form NADPH -H 2 O passes through water-splitting enzyme in PSII to form O 2 or S [Purple and Green bacteria use a single photosystem and don’t use water as an electron donor] Purple Sulfur bacteria -use one chlorophyll (bacteriochlorophyll) - electrons emitted from the photosystem are passed along the electron transport chain, and form a PMF, which is used to make ATP -PMF also used in reverse to form NADH because the electron can’t be raised to a high enough energy level to reduce NAD + Green Sulfur bacteria -use one chlorophyll (bacteriochlorophyll) -electrons emitted from the photosystem can be used to make PMF or NADPH -feradoxin donates e to NADP to form NADPH Chemolithotrophic Anaerobes
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Methanogens (all Archaea not bacteria) e donor H 2 e acceptor CO 2 produces methane gas Chemoorganotrophic Anaerobes Respiratory chain Sulfate reducers e donor H 2 e acceptor S produces H 2 S (swamp odor) or black goo when Fe 2+ present Fermenters Propionibacterium= swiss cheese, acne Phototrophic Anaerobes Purple bacteria Intercellular Sulfur granules e donor H 2 S Green bacteria External sulfur granules
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This note was uploaded on 11/17/2010 for the course BIOL 221 taught by Professor Walter during the Fall '08 term at Purdue University-West Lafayette.

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BIO 221 Exam 2 - E lectron t ransport chain -Prokaryotes...

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