Michaelis-Menten Saturation plot

Michaelis-Menten Saturation plot - [S] (mM) No Inhibitor A...

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Unformatted text preview: [S] (mM) No Inhibitor A (5mM) 0.2 5 3 0.4 7.5 5 0.8 10 7.5 1 10.7 8.3 2 12.5 10.7 4 13.6 12.5 V (mmol/min) Michaelis­Menten Saturation 16 14 12 10 8 6 4 2 0 0 f(x) = 2.23x + 4.71 0.2 0.6 1 1.4 1.8 2.2 2.6 3 3.4 3.8 4.2 0.4 0.8 1.2 1.6 2 2.4 2.8 3.2 3.6 4 4.4 A (5mM) nten Saturation Curve Column B Column C Linear Regression for Column C .6 3 3.4 3.8 4.2 4.6 5 2.8 3.2 3.6 4 4.4 4.8 ) 0.2 0.4 0.8 1 2 4 5 7.5 10 10.7 12.5 13.6 2 3 4 4.3 5 5.5 Michaelis­Menten Saturation 16 14 12 10 V (mmol/min) 8 6 4 2 f(x) = 0.77x + 2.89 0 0 0.20.40.60.8 1 1.21.41.61.8 2 2.22.42.62.8 3 3.23.43. [S] (mM) nten Saturation Curve Column B Column C Linear Regression for Column C .8 2 2.22.42.62.8 3 3.23.43.63.8 4 ] (mM) 1/[S] 5 2.5 1.25 1 0.5 0.25 no inhibitorA 0.2 0.13 0.1 0.09 0.08 0.07 0.33 0.2 0.13 0.12 0.09 0.08 B 0.5 0.33 0.25 0.23 0.2 0.18 0.6 0.5 0.4 1/V (mM/min) Lineweaver­Burk Plot f(x) = 0.07x + 0.17 f(x) = 0.05x + 0.07 0.3 0.2 0.1 0 0 1 2 3 1/[S]mM 4 5 6 f(x) = 0.03x + 0.07 eaver­Burk Plot Column B Linear Regression for Column B Column C Linear Regression for Column C Column D Linear Regression for Column D 3 M 4 5 6 ...
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