Micro lab practical

Micro lab practical - Quiz 1 -Acidic dyes= (+) charge...

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Quiz 1 -Acidic dyes= (+) charge -Basic dyes= (-) charge -Bacteria cell wall= (-) charge Advantages of wet mount -view motility of organism Advantages of Stained Prep. -view morphology of bacteria Disadvantage of Stained Prep. -have to kill bacteria which eliminates motility Simple stain -one stain used Differential stains 1) Gram= differentiates bacteria based on differences in cell wall 2) Acid-Fast Stain= used for waxy celled bacteria that resist dyes Structural stains -staining individual parts of bacteria Negative stain -staining just the background Capsular material= polysaccharides, glycoproteins, polypeptides Capsule= glycocalyx Using modant fixes the dye Using decolorizer differentiates gram + or – Wet mount prep. used to study size, shape, and motility
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Bacteria -prokaryote -flagella -rigid cell wall -multiply by binary fission Protozoa -eukaryote -no cell wall Algae -eukaryote -photosynthetic Fungi -eukaryote -cell wall contains CHITIN -no chlorophyll -non-photosynthetic Yeast single celled, lack mycelium Mold has mycelium Single colony isolation -obtain pure culture -separate one species from another Quiz 2
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Micro lab practical - Quiz 1 -Acidic dyes= (+) charge...

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