ProblemSet9_FA10 - specified by a codon if you knew only...

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Genetics 311 Harris Fall 2010 Problem Set 9 1. The following nucleotide sequence is found on the template strand of DNA. First, determine the amino acids of the protein encoded by this sequence by using the genetic code provided in your text. Then, give the altered amino acid sequence of the protein that will be found in each of the following mutations. Sequence: 3’ TAC TGG CCG TTA GTT GAT ATA ACT 5’ a. mutant 1: A transition at nucleotide 11 b. mutant 2: a transition at nucleotide 13 c. a one-nucleotide deletion at nucleotide 7 d. a T to A transversion at nucleotide 15 e. An addition of TGG after nucleotide 6 f. A transition at nucleotide 9 2. Which anticodon would you predict for a tRNA species carrying isoleucine? State all anticodon possibilities, including those following wobble rules.
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3. a. In how many cases in the genetic code would you fail to know the amino acid
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Unformatted text preview: specified by a codon if you knew only the first two nucleotides? b. In how many cases would you fail to know the first two nucleotides of the codon if you knew which amino acid is specified by it? 4. Explain why mutations in the lac I gene are trans in their effects, but mutations in the lac O gene are cis in their effects. 5. For the genotypes given, fill in the table indicating if, in the presence or absence of lactose, each diploid will produce normal -gal and Permease (assume no glucose present). -galactosidase Permease Genotype No Lactose Lactose No Lactose Lactose I + P + O + Z + Y + /I + P + O + Z + Y + I-P + O c Z + Y-/I-P + O + Z-Y + I-s P + O + Z + Y + /I-P + O + Z + Y + I + P + O + Z-Y + /I-P + O + Z + Y-6. . What happens to cAMP/CAP when: a. High glucose is present: b. Low glucose is present:...
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This note was uploaded on 11/18/2010 for the course BIO 311 taught by Professor Harris during the Fall '10 term at California State University , Monterey Bay.

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ProblemSet9_FA10 - specified by a codon if you knew only...

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