LAB BIO5 - Photosynthesis

LAB BIO5 - Photosynthesis - Victor Cruz Portillo BIO 150...

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Victor Cruz Portillo BIO 150 Photosynthesis 15 Oct. 2010 / 22 Oct. 2010 Estefania Yactayo, Nadile Tousseant, Laetiera Compas, Gisellis Correa Introduction
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Photosynthesis has two main parts, which are the light dependent and the light – independent. In the light-dependent reactions pigments trap energy from light, and this energy is used to split water molecules called photolysis (Anderson, 1966). The light-independent reactions or dark phase of photosynthesis involve the fixing of carbon dioxide (Federer & Tanner, 1966). It makes glucose and fructose chains and also releases oxygen, which passes through the stomata of the plant (King, 2004). Organisms that carry out photosynthesis making their own organic molecules are called autotrophic. Some autotrophic organisms include plants, algae, and blue-green bacteria. Plants have many varieties of pigments, all of which absorb different colors of light, creating starch (Anderson, 1966). Chlorophyll a is the primary plant pigment and makes up about three-fourths of all the plant pigments. It absorbs red and blue light and is not found in photosynthetic bacteria Chlorophyll b is another plant pigment. It absorbs blue-green and orange-red light. Carotenoids are a type of accessory pigment that absorbs blue and blue-green light. These pigments are fat soluble and usually masked by chlorophyll a (Anderson, 1966). Anthocyanin is Chromatography is a process used to separate mixtures that can separate plant pigments (Maitland & Maitland, 2002). Pigments can be separate by paper chromatography by their ability to dissolve in two types of molecules: polar molecules and nonpolar molecules (Maitland & Maitland, 2002). Polar molecules are molecules with covalent bonds that share unequally in electrons. Nonpolar molecules are molecules with covalent bonds that share equally in electrons. Only molecules with the same polarity can dissolve and be attracted to each other (King, 2004). Chromatography paper is a polar substance and the solvent, actetone, is relatively nonpolar
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(Morgan & Brown Carter, 2008). Paper chromatography will be used to wick solvent up to the In the experiment of Pigments in Photosynthesis
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LAB BIO5 - Photosynthesis - Victor Cruz Portillo BIO 150...

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