Foodmicro3 - Lab 3 Clostridium Perfringens and C. Botulinum...

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Lab 3 Clostridium Perfringens and C. Botulinum Clostridum perfringens and C. botulinum can be very harmful food pathogens. The point of this lab is to be become familiar with the different ways to test for this microbe, and what a positive test looks like when one of these microbes is present. After creating an incubating poor plates the results were very minimal. A single colony on plate A of 10 ^ -2 was very large and black, The other colonies on plate B of 10 ^ -2 are small and black with the largest having a slight clear zone around it. The 10 ^ -3 colony is very small. The bacteria were gram (+) rods, and the iron milk test was (+). A positive iron milk test has stormy fermentation and is white and puffy just like our results. A lactose gelatin test showed red gel to liquid gelatinase production turned yellow-orange for acid production with bubbles at the top for gas production, and this indicates a positive test. All of these results indicate that presence of C. perfringens. The low
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This note was uploaded on 04/03/2008 for the course BIOL 2614 taught by Professor Lrlink during the Fall '07 term at Virginia Tech.

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Foodmicro3 - Lab 3 Clostridium Perfringens and C. Botulinum...

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