HW%202%20Fall%20%202010

HW%202%20Fall%20%202010 - 1 of 3 Biological Sciences 102...

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1 of 3 Biological Sciences 102 Fall, 2010 K. Hilt Homework #2 1. Amino acid analysis is done on protein “Z”. The following results are obtained: Amino Acid Number of Residues in Protein “Z” Amino Acid Number of Residues in Protein “Z” A 13 B 13 G 12 W 6 P 5 H 12 Z 24 C 4 K 8 V 24 R 12 S 12 L 23 T 14 Y 12 F 13 NH 4 + 8 a) Calculate the net charge, to the nearest 0.01, of protein “Z” at pH 7. Use the pK a ’s given in homework #1. b) What is the molecular weight of protein “Z”? Use the value of 110 Daltons as an average molecular weight of an amino acid residue. c) At pH 7, would you use a cation exchanger or anion exchange resin to bind protein Z, in order to do ion exchange chromatography? d) Does protein “Z” have quaternary structure? How would you experimentally confirm this? 2. Amino acid analysis of one mole of a peptide yields two moles of methionine, two moles of cysteine, one mole of arginine, and one mole of alanine. Determine the sequence of this peptide from the following information: a) treatment of the intact peptide with FDNB (Sanger’s reagent), followed by acid hydrolysis yields DNP-A; b) treatment of the peptide with cyanogen bromide yields a single
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HW%202%20Fall%20%202010 - 1 of 3 Biological Sciences 102...

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