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pre lab questions - • Five carbon sugar • Phosphate(PO...

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Andrew Platt General Biology I Laboratory Six DNA Structure & Molecular Biology Pre-lab Questions 1. Name the five nitrogenous bases. Which of these bases are purines and which are pyrimadines? Adenine, guanine, cytosine, uracil and thymine. Purines are the double ringed nitrogenous bases: Adenine and Guanine. Pyrimidines are the single ringed bases: Cytosine, Thymine, and Uracil (in RNA) 2. Define Chargaff’s Rule. The proportion of A always equals that of T, and the proportion of G always equals that of C, or A=T and G=C. It follows that there is always an equal proportion of purines (A and G) and pyrimidines. 3. List the components of a nucleotide.
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Unformatted text preview: • Five carbon sugar • Phosphate (PO 4 ) group • A nitrogen-containing (nitrogenous) base. The base may be a purine (adenine, A or guanine, G), a two-ringed structure; or a pyrimidine (thymine, T or cytosine, C), a single-ringed structure. RNA contains the pyrimidine uracil (U) in place of thymine. 4. What term best describes the basic method of replication for DNA? Semiconservative 5. What is meant by "complementary" base pairing? Although the stands are no identical, they each can be used to specify the other by base-pairing. Ex. ATGC-TACG 6. Name the three major steps involved in the replication of DNA. • Initiation • Elongation • Termination Andrew Platt...
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