pre lab questions

pre lab questions - discontinuous manner The 5 to 3...

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Andrew Platt General Biology I Laboratory Seven DNA Isolation & Polymerase Chain Reaction (PCR) Pre-Lab Questions 1. Define the Central Dogma. The nature of information flow in cells was first described by Francis Crick as the central dogma of molecular biology. DNA RNA Protein 2. What makes up the genetic code? Briefly describe stop and start codons. Genetic code consists of a series of blocks of information called codons, each corresponding to an amino acid in the encoded protein. Three codons, UAA, UGA, and UAG, are reserved for another function: they signal “ stop” and are known as stop codons. AUG is used to signal “ start” and is therefore the start codon. 3. What is meant by "leading" and "lagging" strands of DNA? Which one replicates in a
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Unformatted text preview: discontinuous manner? The 5'- to- 3' synthesis of the polymerase and the antiparallel nature of DNA mean that only one strand, the leading strand, can be synthesized continuously . The other strand, the lagging strand, must be made in pieces, each with its own primer. The lagging strand replicates in a discontinuous manner. 4. Where does DNA replication occur? DNA replication occurs at the replication fork. 5. List the three major steps involved in a Polymerase Chain Reaction. PCR amplifies DNA Polymerase used in PCR is Taq derived from Thermus Aquaticus. 1. Denaturation (high temperature) 2. Annealing of primers (low temperature) 3. Synthesis (intermediate temperature) Andrew Platt...
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