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Unformatted text preview: discontinuous manner? The 5'- to- 3' synthesis of the polymerase and the antiparallel nature of DNA mean that only one strand, the leading strand, can be synthesized continuously . The other strand, the lagging strand, must be made in pieces, each with its own primer. The lagging strand replicates in a discontinuous manner. 4. Where does DNA replication occur? DNA replication occurs at the replication fork. 5. List the three major steps involved in a Polymerase Chain Reaction. PCR amplifies DNA Polymerase used in PCR is Taq derived from Thermus Aquaticus. 1. Denaturation (high temperature) 2. Annealing of primers (low temperature) 3. Synthesis (intermediate temperature) Andrew Platt...
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