Exam2-2007 - Name(Last First BIO 361 SOLAR ID FALL 2007...

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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 1 BIO 361 EXAM 2 FALL 2007 NAME:_________________________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has 10 pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Page 8 _______________ Page 9 _______________ Page 10 _______________ Total _______________
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 2 Match the following enzymes with the covalent intermediates they form with their substrates. Each answer may be used once, more than once, or not at all. (1.5 pts each) 1. Chymotrypsin ______ a. a Mixed Anhydride 2. Lysozyme ______ b. a Phosphodiester 3. Carboxypeptidase A ______ c. an Acyl Ester 4. Elastase ______ d. an Acetal Ester Answer each question TRUE or FALSE (1.5 pts each): 5.-9. You have been given two hexasaccharides to be evaluated for cleavage by lysozyme: K. NAG-NAM-NAG-NAM-NAG-NAM L. NAM-NAG-NAM-NAG-NAM-NAG 5. _____ Incubation of both substrates with lysozyme will result in complete degradation to monosaccharides, but they will be cleaved at different rates. 6. _____ Incubation of K with lysozyme is likely to release a disaccharide, but incubation of L with lysozyme may release either a monosaccharide or a trisaccharide. 7. _____ The hexasaccharide K can be bound by lysozyme at least as well as the hexasaccharide (NAG) 6 , but the hexasaccharide L may be less strongly bound. 8. _____ The steric hindrance imposed by two tryptophan residues in the C site of lysozyme distorts the ring of the sugar which binds in the C site into a half-chair in both K and L. 9. _____ Because of the way lysozyme binds substrates, only the NAM ring is distorted in K, and only the NAG ring is distorted in L. 10.-13. The binding of a peptide substrate to carboxypeptidase A results in: 10. _____ The movement of an arginine side chain in the enzyme so that it can donate a proton in the catalytic mechanism. 11. _____ The movement of a glutamic acid side chain in the enzyme so that it can participate in formation of a covalent intermediate with the cleaved substrate. 12. _____ The movement of a tyrosine side chain in the enzyme so that it can participate in a mechanism of acid-base catalysis. 13. _____ The movement of a serine side chain in the enzyme so that it can participate in acylation and deacylation.
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 3 14.-21. You have studied an enyzme which catalyzes the conversion of pine logs into paper,
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This note was uploaded on 11/19/2010 for the course BIO 361 taught by Professor Lake during the Fall '08 term at SUNY Stony Brook.

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Exam2-2007 - Name(Last First BIO 361 SOLAR ID FALL 2007...

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