Exam2-2007Key - _Key_ Name (Last, First) BIO 361 EXAM 2 _...

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________Key __________________________ _____________________________________ Name (Last, First) SOLAR ID 1 BIO 361 EXAM 2 FALL 2007 NAME:___________KEY ________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has 10 pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Page 8 _______________ Page 9 _______________ Page 10 _______________ Total _______________
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________Key __________________________ _____________________________________ Name (Last, First) SOLAR ID 2 Match the following enzymes with the covalent intermediates they form with their substrates. Each answer may be used once, more than once, or not at all. (1.5 pts each) 1. Chymotrypsin ___c___ a. a Mixed Anhydride 2. Lysozyme ___d___ b. a Phosphodiester 3. Carboxypeptidase A ___a___ c. an Acyl Ester 4. Elastase ___c___ d. an Acetal Ester Answer each question TRUE or FALSE (1.5 points each): 5.-9. You have been given two hexasaccharides to be evaluated for cleavage by lysozyme: K. NAG-NAM-NAG-NAM-NAG-NAM L. NAM-NAG-NAM-NAG-NAM-NAG 5. __False___ Incubation of both substrates with lysozyme will result in complete degradation to monosaccharides, but they will be cleaved at different rates. 6. __True___ Incubation of K with lysozyme is likely to release a disaccharide, but incubation of L with lysozyme may release either a monosaccharide or a trisaccharide. 7. __True___ The hexasaccharide K can be bound by lysozyme at least as well as the hexasaccharide (NAG) 6 , but the hexasaccharide L may be less strongly bound. 8. __False___ The steric hindrance imposed by two tryptophan residues in the C site of lysozyme distorts the ring of the sugar which binds in the C site into a half-chair in both K and L. 9. __False___ Because of the way lysozyme binds substrates, only the NAM ring is distorted in K, and only the NAG ring is distorted in L. 10.-13. The binding of a peptide substrate to carboxypeptidase A results in: 10. __False___ The movement of an arginine side chain in the enzyme so that it can donate a proton in the catalytic mechanism. 11. __True___ The movement of a glutamic acid side chain in the enzyme so that it can participate in formation of a covalent intermediate with the cleaved substrate. 12. __True___ The movement of a tyrosine side chain in the enzyme so that it can participate in a mechanism of acid-base catalysis. 13. __False___ The movement of a serine side chain in the enzyme so that it can participate in acylation and deacylation.
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________Key __________________________ _____________________________________ Name (Last, First) SOLAR ID 3 14.-21. You have studied an enyzme which catalyzes the conversion of pine logs into paper,
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Exam2-2007Key - _Key_ Name (Last, First) BIO 361 EXAM 2 _...

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