Exam3-2006Key - BIO 361 EXAM 3 FALL 2006 NAME:_KEY_ ID:_ 1....

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BIO 361 EXAM 3 FALL 2006 NAME:______________KEY _____________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has seven pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Total _______________ Maximum of 100 points
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__________________KEY ________________________ ______________________ Name SOLAR ID# 2 1. Fructose requires several unique enzymes to be metabolized in liver. a. Why does liver need these enzymes, but other tissues do not? (2 points) Liver does not have hexokinase, which phosphorylates fructose. b. Draw the pathway that brings fructose into the glycolytic pathway in the liver cells. Make sure you include all enzymes, intermediates, and cofactors. You do NOT need to use structural formulae. (8 points) 2. Choose the answer from the list below that best matches the following statements concerning pentose metabolism. Only one answer per line. (10 points) __ c __ Hydrolyzes an intramolecular ester. __ e __ Deficiency of this enzyme causes hemolytic anemia. __ b __ Stabilizes carbanion intermediates using a protonated Schiff base. __ a __ Transfers 2-carbon fragments from ketoses to aldoses. __ g __ Converts ribulose-5-phosphate to ribose-5-phosphate a. Transketolase b. Transaldolase c. 6-phosphogluconolactonase d. 6-phosphogluconate dehydrogenase e. Glucose-6-phosphate dehydrogenase f. Ribulose-5-phosphate epimerase g. Ribulose-5-phosphate isomerase Fructose Fructose-1P DHAP + Glyceraldehyde GAP Fructokinase (Mg ++ ) Fructose-1P Aldolase Glyceraldehyde kinase (Mg ++ ) ATP ATP ADP ADP Alcohol deH Glycerol kinase Glycerol-P deH Glycerol Glycerol-3P DHAP NADH NAD + NADH NAD + ATP ADP Alternative pathway
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__________________KEY ________________________ ______________________ Name SOLAR ID# 3 3. Six ATP equivalents are required to convert two molecules of pyruvate to one molecule of glucose. Provide the name of the enzymes catalyzing the reactions requiring ATP (or GTP) in conversion of pyruvate to glucose, and write a balanced equation (substrates, products, cofactors) for the reactions they catalyze. Structures are NOT required. (10 points) Pyruate carboxylase (Biotin): Pyr + HCO 3 - + ATP OAA + ADP + Pi PEP carboxykinase (PEPCK): OAA + GTP PEP + GDP + CO 2 Phosphoglycerate kinase (Mg ++ ) 3-phosphoglycerate + ATP 1,3-Bisphosphoglycerate + ADP
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Exam3-2006Key - BIO 361 EXAM 3 FALL 2006 NAME:_KEY_ ID:_ 1....

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