Exam3-2008 - BIO 361 EXAM 3 Version 1 FALL 2008 NAME:_ ID:_...

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BIO 361 EXAM 3 FALL 2008 Version 1 NAME:_________________________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has 8 pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Page 8 _______________ Total _______________
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 2 1. For each of the following statements, match the most appropriate enzyme from the list below. One answer per line. (2 points each) _____ Utilizes a protonated Schiff base to stabilize a carbanion intermediate. _____ Dissipates a charge separation on a carbonyl by formation of thiohemiacetal at the active site. _____ Requires this “priming” factor to function: 2,3-bisphosphoglycerate. _____ Catalyzes a reversible reaction, but the product has the highest standard free energy of hydrolysis of all intermediates in the glycolytic pathway. _____ Uses the energy in an acyl-phosphate to drive forward the synthesis of ATP. A. Pyruvate kinase B. Aldolase C. Hexokinase D. Phosphofructokinase E. Enolase F. Phosphoglycerate kinase G. Phosphoglycerate mutase H. Phosphoglucose isomerase I. Triose phosphate isomerase J. Glyceraldehyde 3-phosphate dehydrogenase 2. Triose phosphate isomerase catalyzes a reversible isomerization reaction. a. Draw a balanced equation for the reaction catalyzed by triose phosphate isomerase. Include the names and structures of both reactants and products. (4 points) b. Draw the enzyme bound intermediate through which this reaction proceeds. (2 points)
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 3 3. You are doing experiments on glycolysis with a yeast lysate under anaerobic conditions. You have obtained the following data: a. Incubation of the lysate with glucose radiolabeled on a single carbon with C14 results in production of radioactive CO 2 . b. Detailed analysis of the aldolase reaction reveals that the radioactivity is present in the second product that dissociates from the enzyme. Based on this data, which carbon in the glucose is radiolabeled with C14? Draw glucose (in open chain form) and circle the carbon radiolabeled with C14. (6 points) 4. Pyruvate dehydrogenase complex catalyzes an oxidative decarboxylation reaction: a. Draw a balanced equation for this reaction. Include both the names of reactants and products (structures are not required). Include all required cofactors. (4 points) b. Draw the structure of pyruvate and circle the carbon that is removed by decarboxylation. (2 points)
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Exam3-2008 - BIO 361 EXAM 3 Version 1 FALL 2008 NAME:_ ID:_...

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