Exam3-2009 - BIO 361 EXAM 3 Version 1 FALL 2009 NAME:_ ID:_...

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BIO 361 EXAM 3 FALL 2009 Version 1 NAME:_________________________________________________ ID:_____________________________________________________ 1. Write Your name and ID on all pages 2. Write all answers in pen only 3. Make sure your exam has 8 pages including this cover page Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Page 8 _______________ Total _______________
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 2 1. You have discovered a new bacterial aldolase enzyme that can use an 8-carbon bis- phosphorylated sugar as a substrate (drawn below). a. Based on what you know of how aldolase works, draw the structures of the predicted products of the reaction. (4 points) b. Based on what you know of the mechanism for aldolase, circle the product that should dissociate from the enzyme first. (2 points) c. Aldolase catalyzes the cleavage of a carbon-carbon bond. What is the activation barrier that prevents spontaneous cleavage of this bond, and how does aldolase reduce this activation barrier? In your answer, make sure you include the structure of the relevant covalent intermediate. (4 points) CH 2 O- P C=O HO-C-H HO-C-H H-C-OH H-C-OH H-C-OH CH 2 O- P
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 3 2. You are doing experiments on a yeast cell extract under anaerobic conditions. a. You have dialyzed the extract and added back ADP, Pi, ATP, and magnesium. When you add glucose radiolabeled with C14 at the C3 position, two 3-carbon, phosphorylated compounds accumulate. Name and provide structures for these two compounds. (4 points) b. Circle the carbon(s) in the structures above that you predict will be radiolabeled after equilibrium. (3 points) c. You performed the same experiment on non-dialyzed yeast extract under anaerobic conditions. Name and provide structures for the final products of the metabolism of glucose under these conditions, and circle the carbon(s) that would be radiolabeled. (3 points) 3. You are now doing experiments with a mitochondrial extract under aerobic conditions. Malate radiolabeled with C14 at a single carbon is incubated with the mitochondrial extract. You observe that radioactive carbon dioxide is released at the same time that alpha- ketoglutarate is formed. Draw the structure of Malate and circle the carbon that was labeled with C14. (6 points)
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__________________________________ _____________________________________ Name (Last, First) SOLAR ID 4 4. For the pyruvate dehydrogenase reaction: a. Draw a balanced equation for the reaction catalyzed by this multi-enzyme complex. Structures are not necessary. (3 points)
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This note was uploaded on 11/19/2010 for the course BIO 361 taught by Professor Lake during the Fall '08 term at SUNY Stony Brook.

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Exam3-2009 - BIO 361 EXAM 3 Version 1 FALL 2009 NAME:_ ID:_...

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