Exam4-2009Key - BIO 361 EXAM 4 AND CUMULATIVE FINAL VERSION...

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BIO 361 EXAM 4 AND CUMULATIVE FINAL FALL 2009 VERSION 1 NAME:________KEY _____________________________________ ID:_____________________________________________________ INSTRUCTIONS FOR EXAM 4 (pages 2-7): 1. Write your name and ID on pages 1-7 2. Write all answers in pen only 3. Limit your answers to the space provided Page 2 _______________ Page 3 _______________ Page 4 _______________ Page 5 _______________ Page 6 _______________ Page 7 _______________ Total _______________ Maximum of 100 points INSTRUCTIONS FOR CUMULATIVE FINAL (pages 8-16) (100 points total): a. Answer all questions on the Opscan sheet. Only one answer per question. b. Use a number 2 pencil. c. Fill in NAME (LAST, FIRST, and MI), and IDENTIFICATION NUMBER (Solar ID number) using the bubbles. d. Fill in bubble “1” (for Version 1) in the first column under DAY (do not fill in the remainder of BIRTH DATE).
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KEY _______________________________ _____________________________________ Name (Last, First) SOLAR ID 2 1. Fatty acid synthesis a. Fill in the table below describing the differences between fatty acid (FA) degradation and synthesis in terms of subcellular location(s), redox potential donor(s)/acceptor(s), and intermediate carriers. (6 points) FA degradation FA synthesis Subcellular location(s): Mitochondria Cytosol Redox donor(s)/acceptor(s): FAD/NAD + NADPH Intermediate carrier(s): CoA ACP b. Name the final product of the mammalian fatty acid synthase. (2 points) Palmitate c. Name two physical/structural features that enable a tethered substrate to reach spatially distinct enzymatic motifs in the mammalian fatty acid synthase complex. (2 points) Lengthy, flexible tether (2 nm) (Phosphopantetheine) Centrally located ACP Large structural or conformational changes of the complex (Any 2 of the 3 answers earn 2 points) 2. Cholesterol Biosynthesis a. Describe the four stages for cholesterol biosynthesis. Provide names for the beginning substrate and end products for each stage (structures are not required). (8 points) (1) Synthesis of isopentenyl-pyrophosphate (C5) from Acetyl-CoA. (2) Condensation of 6 isopentyl-pyrophosphates to make squalene (30C). (3) Cyclization of squalene to make lanosterol. (4) Modifications of lanosterol to make cholesterol. b. Name the enzyme that catalyzes the rate-limiting (or committed step) for cholesterol biosynthesis. (2 points) HMG CoA reductase
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KEY _______________________________ _____________________________________ Name (Last, First) SOLAR ID 3 3. Photosynthesis and Calvin Cycle
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Exam4-2009Key - BIO 361 EXAM 4 AND CUMULATIVE FINAL VERSION...

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