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Prob%20Fest%2010-1-10 - A What is the pH of a buffer that...

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Unformatted text preview: A. What is the pH of a buffer that performs as follows? To 4 5 ml of the buffer is added 0.5 rnl of 0.5 mM pr02 phenol and the A410 measured rs O 300 OH {/tfié/ in: M’vfimwfl fVav/z’i I/I/ LUZ/fl [/3 M Zip/Jag cw Gm: {MHZ/clam” PK 7 2« MM 6410 : 232- 700M ”7% A €0»300 W CC (PI/62, firm/10‘); )3} 100 M"! W C(’)3Ax5’fip)12)20'”) 7—; /; (3sz /a_§/\/Z 5.5M - 7712 7129ng WAWMW ‘5 P ”3’7 WM 7 ‘9 MK”; 5 ,x lo 3M) :— (i: OSXIO €33M E “ 5 00% /U”>/Vl mel’iucémw/LMw M0 /9//)e,MC/L/: m (5 00 /éo)x/c>5/L/ P 2 ~= 5 77' (Cl “gm Um pat/L We alt HI/ )2; euduafi I’Zw, pH pH?“ 7. [5 I /cj (1%) N a -"\ 7/5 f/éf‘jé 47/) 7 /5‘*@ 33 =5? B What 15 the total buffer concentration of a pH 6 8 buffer with a pKa of 6 5 that performs as follows: 1. 00 mL of O 05 N HC111S added to 4. 00 mL of the buffer and the pH 1snow6u2 , N ,, B1Qi8;:w 1%M%%%hfiyf éfl;kfié BIL/L ,: 1 ~ /‘ 11:12:1/ x1 {1 1g 15g- 3 H {1’sz 1 1 H fig: f 1mg): I1“ 77er 5 1146/; 408 Bi/BHZ. 62:11-96 X‘] M 518/ M m9 21111221de Suzy/3 fldéezmé ”6 [/15ch 511%»: /11M MW 4’ C609 1/1111 /Lé béfiagfafimfliHvawfl 6482, 81111111 '11 1111 11517320111, 1/11/1111 1111111121 161 057/33" (JO/H '=§%=035H700N4 , 2%; QMJWWQZ $5 7L/07 /}“‘f “0% k Q/j _ }/_ 107%: M0138; «(210224 2” g G 38211 10.11/‘4 11M 033/1 11002111 21B 111*» (JR/VI 11113111113 03M ~ /1 B: 1 0 613/1 11mg? 134/ '7 Bf/Z DOW/1137108122.) Mgfvc-agj 2* i2 B 21114 1: 012111: B2“ /25m1~B [21511211 $2141 C What 15 the concentration of Tris 111 a pH 8 0 buffer if 4 0 m1 of the buffer rs treated with lmL of 0.05 N HC1 and the resulting pH 15 2 5? P1 I: 21g “’3 E1113; 3111:1011 M K _ 5 V : ‘119 “111:1 LVHjE) (1671/3 1011/3123 6€\13u1/[email protected]@ Vurafi E1113; : 11 01113 1M1“ 11%; mm (53316 (@0510) ” ~zm1uxamm 1,8,1: T1);_ 1’ WM 3/1/16: 1% W13 1061/14 (L 1T1: Om 1:) Lj’flgé/L W T113: 0 8T ’2 TOM/11’1" 3 119 W1 111/1: 911,511? 1/1/1111 ,V1ffiwwfi “33113111 111111 'b-LT‘ = 335 M11 771111111 WW/wj 511% 1:1 8.0 / 011111 80 8.2,} +19 35% ~021=10q&§%v ‘ D. What is the room temperature if to bring the Tris buffer prepared in experiment 01 to pH 8.0 required only 5.4 mL of 1.0 N HCl? , 7 ram —S‘W~€ {“3 '2 PKa (RT) "i7 10? ‘34 Mé : pKQCIZT) + ieflggz) 3,0? : PKMEJ') NW 9W1 pk} [kHz in; 5x905} {m ’D/is mg (202) "= 5‘2? [3% (5??) 1‘ “gang [>qu (30% ‘27) 3 044 Pm ”r X APKA GT?) ': 0:449 {1Q "f 1;“; Ejz/LC’i/fifié W +£4W?’0 +2} QT gran/A 2.0 >< =(”‘ “6% 3072) (Became) :5. 251:1 is RT (9% W4 via/x} bum ZO°L+9ZZ= ZEC’C, , ...
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