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Prob%20Fest%2010-15-10 - A 82800711 = 5700 M“cm"...

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Unformatted text preview: A 82800711) = 5700 M“cm" 8230(Y) = 1500 Mlcm" A 1.0 mg/ml solution of lactate dehydrogenase (LDH) has an A230 = 1.20. The molecular weight determined by SDS gel electrophoresis is 36,400. 1.) Determine the molar extinction coefficient of LDH at 280 nm. This 1.0 mg/ml solution of LDH was treated with a 10X excess of tetranitromethane resulting in a new absorption at 430 nm with an A430 = 0.789. Tetranitromethane selectively nitrates tyrosine sidechains resulting in a new band with a mm = 430 nm with a molar extinction coefficient of 4100 M'lcm‘]. 2.) Determine the number of tyrosines in LDH. 3.) With this information estimate the maximum number of tryptophans in LDH. : a: "e 2393 5510604 4% 3.7.0 : r32 ’2, arms“ QM W . 5W4 25“" 4.3%(35‘; W; W” M ti iii 3 7 r $3212: x':3 M MM 44525205.”in M M3 g S 7‘ l L? 3‘3 :4 23>? LE”! eat/'8‘ . ' 5;; «0 i “'2‘ ‘ . g‘ ’4 as, T AW 21W 0 33559 a w... ' %3E M53” 3. mfiwweewm ,3 1" 575550 ‘ :3. 8280(W) : 5700 Mlcm" mm = 1500 M'1cm-1 A 0.5 mg/ml solution of lysozyme has an A230 = 1.32. The molecular weight determined by SDS gel electrophoresis is 14,300. 1.) Determine the molar extinction coefficient of lysozyme at 280 nm. This 0.5 mg/ml solution of lysozyme was treated with a 10X excess of tetranitromethane resulting in a new absorption at 430 nm with an A430 = 0.430. Tetranitromethane selectively nitrates tyrosine sidechains resulting in a new band with a kmax = 430 nm with a molar extinction coefficient of 4100 M'Icm'l. 2.) Determine the number of tyrosines in lysozyme. 3.) With this information estimate the maximum number of tryptophans in lysozyme. C 3280010 = 5700 M'lcm'l 328000 = 1500 M‘cm" A 1.0 mg/ml solution of alkaline phosphatase has an A280 — l. 39. The molecular weight determined by SDS gel electrophoresis IS 99 500. 1.) Determine the molar extinction coefficient of alkaline phosphatase at 280 nm. This 1 0 mg/ml solution of alkaline phosphatase was treated with a 10X excess of tetranitromethane resulting 1n a new absorption at 430 nm with an A430— — l 64. Tetranitromethane selectively nitrates tyrosine sidechains resulting 1n a new band with a Kmax— — 430 nm with a molar extinction coefficient of 4100 M cm . 2.) Determine the number of tyrosines in alkaline phosphatase. 3.) With this information estimate the maximum number of tryptophans in alkaline phosphatase at; E 3 /~ 3%”x’fafi M 1:] 1 E .. v,» . 5 t , , rxu 2 .v‘. A” >11 r v b ,w v 1 Izg .. , y F Lita) ”Wig REM '2 8230(W) = 5700 M'lcm" mm = 1500 M'Icm'l A l .0 mg/ml solution of BSA has an A280 — 0. 667 The molecular weight determined by SDS gel electrophoresis is 66,300. 1» 2'5; #515) as,“ 1.) Determine the molar extinction coefficient of BSA at 280 nm. 2' '1 - This 1.0 mg/ml solution of BSA was treated with a 10X excess of tetranitromethane resulting in a new absorption at 430 nm with an A430 — l 18 Tetranitromethane selectively nitrates tyrosine sidechains resulting 1n a new band with a kmax— — 430 nm with a molar extinction coefficient of 4l00 M cm ~ ,1 2.) Determine the number of tyrosines in BSA. i ‘ é ‘ it " \. r3“ 3.) With this information estimate the maximum number of tryptophans in BSA ...
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